An audio message purportedly from Isil warned has that a Japanese hostage has less than "24 hours left to live" if the jihadists' demands are not met.
The message, posted online yesterday afternoon, also threatens the life of Jordanian pilot Mu'ath al-Kaseasbeh - another hostage of Isis - whom it warns has "even less" time.
The recording again demanded the release of Sajida al-Rishawi, an Iraqi woman sentenced to death in Jordan for involvement in a 2005 terror attack that killed 60 people.
Yesterday's audio message showed a still photograph of Kenji Goto holding an image of Mr Kassasbeh.
It was entitled 'The second public message of Kenji Goto Jogo to his family and the government of Japan'.
In the recording, the person claiming to be Mr Goto says: "I have been told this is my last message".
The format matched a message released over the weekend, though the accompanying image did not bear the logo of Isil's al-Furqan media arm.
Several militant websites affiliated with Isil referenced the recording and posted links to it late yesterday.
A Japanese envoy in Jordan, deputy foreign minister Yasuhide Nakayama, earlier expressed hope the two hostages would return home "with a smile on their faces".
"I hope we can all firmly work hard and join hands to cooperate, and for the two countries [Japan and Jordan] to co-operate, in order for us to see the day when the Jordanian pilot and our Japanese national Mr Goto, can both safely return to their own countries with a smile on their faces," he told reporters late on Monday night after another day of crisis talks in the Jordanian capital. (© Daily Telegraph, London)