FC Porto will have to end Juventus's 46-game unbeaten run at home to have any chance of qualifying for the last eight of the Champions League.
The Italian champions haven't tasted defeat at home since August 2015 but the Portuguese, who start the tie 2-0 down, have in impressive away record of their own to give them hope, with four wins and a draw in the last five games.
Juve forward Paulo Dybala is also wary of the inspiration last week's Barcelona 6-1 victory over PSG has given all sides facing a mission impossible.
"Barcelona gave confidence to every team that are behind with their incredible come-back but honestly not every team have the quality that Barcelona possess," said the Italian, who is already thinking of the final in Cardiff.
"I have team-mates who want to win this trophy really badly and some others that have already won it and want more. Going all the way in this competition is the only things that really counts."
Juventus's Giorgio Chiellini has recovered from a thigh injury while Mario Mandzukic (illness) and winger Juan Cuadrado (suspension) are available again.