The World Anti-Doping Agency (WADA) has revoked the accreditation of the drug-testing laboratories in Kazakhstan's Almaty and Bloemfontein in South Africa.
Both labs have been suspended for the last year and have therefore not been conducting drug tests - but now lose their WADA status entirely for failing to meet the required standard.
The sanctions will come into force within 30 days of notification and if they wish to appeal against the decisions they must notify the Court of Arbitration for Sport within 21 days.
The Almaty and Bloemfontein labs become the second and third drug-testing centres to be punished in this way in less than two years, following the stripping of Moscow lab's accreditation in November 2015 for its part in Russia's state-sponsored doping programme.
This still leaves 32 WADA-accredited labs worldwide, although the facilities in Bogota, Los Angeles, Lisbon and Mexico City are currently suspended and under review.
In a statement, WADA said it was "responsible for accrediting and re-accrediting anti-doping laboratories, thereby ensuring that they maintain the highest quality standards".