The number of cases of Chlamydia in the capital has increased by almost a third, according to new figures released by the Dublin Well Woman Centre.
In their annual report for 2014, 253 positive cases of the infection were recorded - an increase of 32pc compared to figures for 2013.
This is the second highest recorded figure in the last 13 years.
The number of people presenting themselves for testing also increased by 20pc.
The highest ever level of tests occurred last year with 5,042 tests conducted across the organisation's three Dublin clinics.
Alison Begas, chief executive of the Dublin Well Woman Centre said despite increased awareness around sexual health, the number of Sexually Transmitted Infections (STIs) are increasing at an alarming rate.
"These results highlight the need to expedite publication of the National Sexual Health Strategy," she said.
"The Well Woman Centre takes a proactive approach in promoting the availability and importance of testing, however we are only one service provider, and access to standardised testing must be improved nationwide.
"These diseases are becoming more prevalent, and we need action from the HSE now," she added.
The report also shows an increase in the number of women choosing long-acting reversible contraception (LARCs).
For the first time, more than 1,000 women had LARCs fitted with a total of 1,117 women choosing this form of contraception.
A worrying trend in the report was the decline in women attending their free cervical screening tests.
Just 7,198 were tested last year, down from a high of almost 10,000 tests in 2009.
However, the greatest fall in numbers for smear tests is in the 25-29 age group, which may be linked to emigration.