The video was broadcast before the Facebook Town Hall Q & A yesterday.
Zuckerberg showed viewers around the headquarters of Facebook in what one commenter called "MTV Cribs, Facebook style".
Zuckerberg showed the camera his desk, which was neatly organised with a laptop, a number of books which he was reading for his 'year of books challenge' and a piece of designed art with the updated Facebook logo.
"In Facebook no-one has offices. I don't sit at my desk, it's not where I spend most of my time. I do have a conference room for meetings".
Explaining the glass walls, he said "We want to create an open and transparent culture at Facebook where everyone can see what everyone else is working on to enable us all to do our best work".
Reaction to the livestream was positive and commendations flooded in about the quality.
First live video at Facebook HQPosted by Mark Zuckerberg on Monday, 14 September 2015
One commenter wrote that the "iOS experience is off the chain", while another added "very high Weissman score for this livestream".
The video, which is now available on Zuckerberg's Facebook page for replay, has been viewed over five million times.
Facebook joins Periscope, Meerkat, YouNow and YouTube for services offering live-streaming capabilities.