The state's high court in Los Angeles said it would deny an appeal by the actress in her wrongful termination case against the ABC network and Touchstone Television.
Nicollette said she was fired because she claimed she was hit in the head by series creator Marc Cherry.
ABC lawyers claimed her contract simply was not renewed.
In March, jurors were deadlocked after a two-week trial. An appeals court found in August that Nicollette was not entitled to a new trial, but should be allowed to file an amended lawsuit claiming she was retaliated against because she complained about her boss.
Desperate Housewives concluded its eight-season run this year.