RTE's religious affairs show 'The God Slot' sparked a furious backlash after tweeting: "Can gays be cured of being gay?"
The state broadcaster moved to apologise and insisted the tweet was to "grab people's attention" as opposed to offering a stance on the issue.
The controversy began when 'The God Slot' tweeted to promote the radio programme at 10 o'clock tonight.
The tweet asked: "Can gays be cured of being gay? 'Try The God Slot'."
The Twitter account of the show, presented by newsreader Eileen Dunne, was immediately inundated with angry comments, while the Gay and Lesbian Equality Network branded the tweet "offensive".
The show initially responded to the hostile reaction by telling people to "listen to the programme before judging".
However, the tweet was later deleted, and another comment posted to apologise.
"Apologies if offended by a promo for @TheGodSlot tomorrow night. The contributor refutes the value of any conversion therapy for gay people," it read.
An RTE spokeswoman said that the programme tonight investigates an organisation which is claiming to cure gay people.
She said an undercover journalist investigated the organisation and exposed the claims as "ridiculous".