The temporary exhibition charts the rise of formidable matriarch and comedy character Agnes Brown a woman who, according to O'Carroll, shares many similarities with his late mother and former Labour TD Maureen.
"Mrs Brown is my mother without the education," he told the Irish Independent. "If Agnes had the education my mother had she would have been a TD not a Moore Street trader."
A picture of a nine-year-old Brendan standing beside his mother provoked the most emotional response from the comedian.
The image was taken the morning of O'Carroll's ninth birthday, later that day he would appear in court for stealing a bicycle bell and roll of Sellotape from Superquinn.
"I was sent to Borstal the next morning. It's the only picture I have of the two of us so it was painful seeing it but also lovely.
"I wish she was still here to see all this but she'd be 107 this year so she probably wouldn't have a clue what was going on," he laughed.
Pieces of the 'Mrs Brown's Boys' set, ephemera from early stage shows, the infamous fat suit and stories from O'Carroll's life cover the walls of the museum.