Kristen Stewart's finale as Bella in The Twilight Saga: Breaking Dawn - Part 2 was number one again with 64 million dollars (£40 million) during the five-day holiday stretch that began on Wednesday, according to studio estimates.
Daniel Craig's Bond adventure Skyfall came in at number two with 51 million dollars (£32 million), while Daniel Day-Lewis and Steven Spielberg's Civil War saga Lincoln finished third with 34.1 million dollars (£21 million).
This Thanksgiving was also a huge 25 per cent jump from a year ago, when domestic revenues were a weak 232 million dollars (£145 million) as some big holiday releases fizzled.
With a strong December line-up ahead, Hollywood has resumed its record revenue pace for the year after a brief box-office lull in late summer and early autumn.
Domestic revenues for 2012 are at 9.75 billion dollars (£6 billion), putting Hollywood potentially on track for its first 11 billion-dollar (£7 billion) year, which would beat the 2009 record of 10.6 billion dollars (£6.6 billion), said Hollywood.com analyst Paul Dergarabedian.
The Twilight finale, Skyfall and Lincoln finished in the same top-three rankings for the second-straight weekend as new releases were unable to dislodge them.
Leading the newcomers was Paramount and DreamWorks Animation's animated tale Rise Of The Guardians at number four with 24 million dollars (£15 million) for the weekend and 32.6 million (£20.3 million) since opening on Wednesday.