Photographs and previously unseen diary extracts charting a royal visit to Bethlehem 150 years ago are to go on display.
He was joined by Francis Bedford - the first photographer to accompany a royal tour.
Bedford's images of the trip captured a view of Bethlehem from the roof of the Church of the Nativity, said to be built on the spot where Jesus was born.
He also took a picture of the Shepherds' Field showing the area where the Angel Gabriel is said to have appeared to the shepherds.
The photographs, which belong to the Royal Collection, will form part of a new exhibition Cairo to Constantinople: Early Photographs of the Middle East at The Queen's Gallery, Palace of Holyroodhouse in Edinburgh, from March 8, 2013.
Curator Sophie Gordon said the purpose of taking the photograph from the Church of the Nativity was to show the Victorian audience that Bethlehem really existed and to add weight to the Christian tradition.
"Bethlehem wasn't directly on the Prince's route - and so his party made a particular point of going there," she said.
Dr Gordon added: "Very little was known about this part of the world at the time, and what information they did have was largely based on knowledge of the Bible."
In the early 1860s, photography was still in its infancy and had only been introduced to the public in 1839.